|
Type: Recombinant |
Cat. No.: 41040 |
|
Tag: His |
Size: 0.1 mg |
|
Source: E.coli |
Purity: >95% |
|
Other names: E-FABP; PA-FABP |
Species: Human |
Introduction
The fatty acid binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as
eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular
membranes. The fatty acid binding protein 4 (FABP-4) and fatty acid binding protein 5(FABP5) are closely related and both are
expressed in adipocytes. Mice with targeted disruption of FABP-4 accompany FABP-5 almost completely protect against
diet-induced obesity, insulin resistance, dyslipidemia, type 2 diabetes, and fatty liver disease. While mice over expressing FABP5 in
adipose have reduced insulin sensitivity.
Description
Total 162 AA. Mw: 18.4 kDa (calculated).
N-terminal His-tag and TEV cleavage site, 28 extra AA (highlighted).
Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSATVQQLEGRWRLVDSKGFDEYMKELGVGIALRKMGA
MAKPDCIITCDGKNLTIKTESTLKTTQFSCTLGEKFEETTADGRKTQTVCNFTDGALVQHQEWDGKE
STITRKLKDGKLVVECVMNNVTCTRIYEKVE
Formulation
Filtered (0.22 μm) and lyophilized in 1 mg/mL in PBS.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Storage
Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Application
Western blotting.
